Thermo Fisher Scientific CTCF Recombinant Rabbit Monoclonal Antibody (JM10-61)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Immunoprecipitation (IP)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM10-61

Immunogen

Synthetic peptide within Human CTCF aa 685-727 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CTCF,uniProtId:P49711-1,ncbiNodeId:9606,antigenRange:685-727,antigenLength:727,antigenImageFileName:MA5-32640_CTCF_P49711-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32640_CTCF_P49711-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809917

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms` tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.