Thermo Fisher Scientific Transferrin Receptor Monoclonal Antibody (H68.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 320 publications 320 publications

Immunohistochemistry (IHC)

-

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:250

View 100 publications 100 publications

Flow Cytometry (Flow)

-

View 10 publications 10 publications

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 16 publications 16 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Control (Ctrl)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

Assay-dependent

View 1 publication 1 publication

GST Pull Down (GPD)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 87 publications 87 publications

Product Specifications

Species Reactivity

Chicken, Hamster, Human, Mouse, Rat

Published species

Bovine, C. elegans, Cat, Dog, Hamster, Horse, Human, Insect, Mammal, Mouse, Non-human primate, Pig, Rat, Virus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

H68.4

Immunogen

Recombinant human transferrin receptor.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533029

Product Specific Information

H68.4 is specific to residues 3-28 of the human transferrin receptor (TfR) tail.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.