Thermo Fisher Scientific Spectrin beta-4 Monoclonal Antibody (N393/2), FITC
FITC 형광 표지된 Spectrin beta-4 단클론 항체로 인간, 마우스, 랫트 시료에 반응합니다. WB, IHC, ICC/IF 등 다양한 응용에 적합하며, 단백질 G 정제 및 보존제 무첨가로 고순도 유지. 4°C 암소 보관.
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Thermo Fisher Scientific Spectrin beta-4 Monoclonal Antibody (N393/2), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N393/2 |
| Immunogen | Fusion protein amino acids 1621–1832 (C-terminal repeats 14–15) of human Beta4-spectrin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932176 |
Additional Formats
- Unconjugated: MA5-27608
- APC conjugate: MA5-45721
- PE conjugate: MA5-45724
- PerCP conjugate: MA5-45723
- Custom conjugation available upon request
Product Specific Information
- A 1:100 dilution of MA5-45722 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately >200 kDa.
- Does not cross-react with other Beta-spectrins.
- This antibody was formerly sold as clone S393-2.
Target Information
Spectrin is a major component of the cytoskeletal network underlying the erythrocyte plasma membrane, determining cell shape and organization of transmembrane proteins and organelles.
Spectrin beta 4 is a non-erythrocyte member expressed primarily in the brain and pancreatic islets, localized to the nuclear matrix, cytoplasmic vesicles, and PML nuclear bodies.
Beta4 spectrins are essential for membrane stability and organization of the nodes of Ranvier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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