Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, FITC
Rabbit anti-Rat IgG (H+L) FITC 결합형 2차 항체로, 면역세포염색(ICC/IF) 및 유세포분석(Flow Cytometry)에 적합합니다. 친화 크로마토그래피로 정제되었으며, 높은 특이성과 감도를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535691 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Conjugate Formats
- AP
- Biotin
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified and highly specific for rat immunoglobulins. They are useful for detection, sorting, or purification of rat antibodies.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
These antibodies are typically produced by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and optional modification such as antibody fragmentation or label conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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