Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Rabbit anti-Rat IgG (H+L) AP 결합 2차 항체로 Rat IgG 검출에 사용됩니다. WB, IHC, ELISA 등 다양한 응용에 적합하며, 높은 특이성과 감도를 제공합니다. Affinity chromatography로 정제된 액상 형태로, 장기보관 시 -20°C에서 보관합니다.
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Applications 및 Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535693 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is verified by immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, or label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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