Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biotin
Thermo Fisher Scientific의 Rabbit anti-Rat IgG (H+L) Biotin 결합 2차 항체. Rat IgG 검출용으로 Western blot, ICC/IF, Flow cytometry, ELISA에 적합. 높은 민감도와 특이성, ELISA 및 IEP로 품질 검증 완료. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535694 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of antibody is confirmed for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG targets. Secondary antibodies offer versatility for various detection systems (HRP, AP, fluorescence) and enhanced sensitivity through signal amplification. Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, these antibodies may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to achieve high specificity.
Additional Formats
- [AP]
- [FITC]
- [HRP]
- [Texas Red]
- [TRITC]
- [Request custom conjugation]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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