Thermo Fisher Scientific CD16 Chimeric Recombinant Rabbit Monoclonal Antibody (3G8)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3G8

Immunogen

3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD16,uniProtId:P08637-1,ncbiNodeId:9606,antigenRange:1-254,antigenLength:254,antigenImageFileName:MA5-48068_CD16_P08637-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48068_CD16_P08637-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:CD16,uniProtId:O75015-1,ncbiNodeId:9606,antigenRange:1-233,antigenLength:233,antigenImageFileName:MA5-48068_CD16_O75015-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48068_CD16_O75015-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074511

Product Specific Information

Specificity: 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.