Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Monoclonal Antibody (CPTC-HSPB1-2)
Heat Shock 27kDa Protein 1 (HSPB1/HSP27)에 특이적인 Mouse monoclonal antibody. WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. 200 µg/mL 농도의 Liquid 형태로 제공되며, Protein A/G 정제. 세포 스트레스 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-HSPB1-2 |
| Immunogen | Recombinant human full-length HSP27 (HSPB1, UniProt ID: P04792-1, amino acids 1–205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
In response to environmental stress, cells increase expression of heat shock or stress proteins.
HSPB1 (Heat Shock Protein Beta-1, also known as HSP27) is a small heat shock protein functioning as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
It contributes to stress resistance and actin organization, regulating processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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