Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Monoclonal Antibody (CPTC-HSPB1-2)
Heat Shock 27kDa Protein 1(HSPB1/HSP27)에 특이적인 mouse monoclonal antibody. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 단백질 A/G로 정제된 액상 형태로 제공되며, 4°C에서 보관. 세포 스트레스 반응 및 단백질 폴딩 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-HSPB1-2 |
| Immunogen | Recombinant human full-length HSP27 protein (UniProt ID: P04792-1; antigen range: 1–205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
HSPB1 (Heat Shock Protein Beta-1, also known as HSP27) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization. Through its chaperone activity, HSP27 regulates various biological processes including phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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