Thermo Fisher Scientific HSP27 (Heat Shock Protein 27) Monoclonal Antibody (HSPB1, 774)
HSP27 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 인간 및 여러 종에 반응하며, 단백질 A/G 정제 및 액상 형태로 제공. 스트레스 반응 및 세포 보호 연구에 적합.
- 카탈로그번호
- 3315-MSM2-P0
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Thermo Fisher Scientific HSP27 (Heat Shock Protein 27) Monoclonal Antibody (HSPB1, 774)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Chimpanzee, Human, Mouse, Non-human primate, Sheep, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HSPB1, 774 |
| Immunogen | Recombinant human HSPB1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
In response to adverse environmental changes, cells increase the expression of heat shock or stress proteins. HSPB1 (Heat Shock Protein Beta-1, also known as HSP27) is a small heat shock protein functioning as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates various biological processes including phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene can result in Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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