Thermo Fisher Scientific H3K18ac Polyclonal Antibody
H3K18ac에 특이적인 Rabbit Polyclonal Antibody로 다양한 종에서 반응성을 보이며 WB, IHC, ICC/IF, ChIP, Dot blot 등에 사용 가능. 고순도 Affinity chromatography로 정제된 액상 형태로 안정성 높음. 연구용으로만 사용.
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- 600-401-I81
- 판매단위
- pk
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Thermo Fisher Scientific H3K18ac Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [ac Lys18] affinity purified antibody prepared from whole rabbit serum immunized with a synthetic acetylated peptide surrounding Lysine 18 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H3 [ac Lys18] reacts with human Histone H3.2. BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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