Thermo Fisher Scientific H3K18me3 Polyclonal Antibody
H3K18me3 특이적 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF, ChIP, Dot blot 등에 사용 가능. 다양한 종(C. elegans, Human 등)에 반응하며, 고순도 친화 크로마토그래피 정제 제품. -20°C 보관, 연구용 전용.
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Thermo Fisher Scientific H3K18me3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Trimethyl Lys18] affinity purified antibody prepared from whole rabbit serum immunized with synthetic trimethylated peptide surrounding Lysine 18 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Histone H3 [Trimethyl Lys18] reacts with human Histone H3.2.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes.
Each nucleosome consists of ~146 bp of DNA wrapped around an octamer of core histones (H2A, H2B, H3, H4).
Chromatin is further compacted by linker histone H1.
This intronless gene encodes a member of the histone H3 family, found in a histone cluster on chromosome 1.
Transcripts lack polyA tails and contain a palindromic termination element.
This record represents the telomeric copy of duplicated histone genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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