
Thermo Fisher Scientific SMARCB1 Polyclonal Antibody
SMARCB1 단백질 검출용 토끼 폴리클로날 항체. Western blot과 면역세포화학(ICC/IF)에 적합. 인간, 생쥐, 제브라피시에 교차 반응 가능. 액상 형태로 제공되며 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 276–288 of human SNF5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2191702 |
Product Specific Information
- Recommended positive control: HeLa nuclear extract
- PA1-25450 is expected to cross-react with mouse (100% conserved) and zebrafish (100% conserved) due to sequence homology.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The SWI-SNF complex is involved in transcription activation through ATP-dependent nucleosome remodeling. Brm (SNF2alpha) and Brg-1 (SNF2beta) are ATPase subunits of this complex. Core components include Brm, Brg-1, Ini1 (SNF5), BAF155 (SRG3), and BAF170. Addition of Ini1, BAF155, and BAF170 enhances remodeling activity, while other subunits may have regulatory roles. hSNF2L and hSNF2H are homologs of Drosophila ISWI, present in other chromatin remodeling complexes such as NURF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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