Thermo Fisher Scientific AmpFLSTR Identifiler Direct PCR Amplification Kit, 1000 Reactions

Applied Biosystems™ AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit

The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of 16 loci included in the Identifiler Kit, eliminating the need for DNA extraction or purification steps for DNA database and other single-source reference samples.

Blood or buccal samples on FTA Cards can be directly punched into PCR plates or tubes for amplification without reduction in data quality. Samples collected with the Buccal DNA Collector and other non-FTA substrates can be processed without heat incubation or additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features

• Direct amplification of blood and buccal samples without extraction or purification
• High-throughput and automated sample processing
• Reduced contamination risk and procedural errors
• Faster results with maintained data quality
• Lower extraction/purification and labor costs

The Identifiler Direct Kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler Kit, which:

• Maximizes concordance with STR profiles from other AmpFLSTR kits (Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus, COfiler)
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Performance Data (Table 1)

As part of its rigorous development, the Identifiler Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess “first pass” success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA Cards and Buccal DNA Collectors were amplified with the Identifiler Direct Kit and analyzed using GeneMapper ID-X at two different minimum peak amplitude thresholds.

The Confidence to Do More

The Identifiler Direct Kit is part of a new generation of AmpFLSTR Kits that harness significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence. Laboratories using this kit can significantly expand throughput while ensuring sample integrity and quality assurance.

Complete Validated Solution

This kit is part of a comprehensively validated system for STR-based Human Identification. PCR reaction components, primer sequences, and amplification protocols have been optimized and validated with Applied Biosystems instruments and software to provide robust amplification. Validation experiments were performed according to DAB and SWGDAM guidelines. Each laboratory should perform its own internal validation studies.

The kit is manufactured under ISO 9001 quality system requirements. A Certificate of Analysis is available upon request.

Experience Matters

Applied Biosystems designs and validates reagents, instruments, and software as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available for support and troubleshooting.

Note: For Forensic or Paternity Use Only.

Important Notice

Purchase of this product conveys a non-transferable license for forensic or paternity testing only. It does not include rights for chimerism determination, cell line authentication, cancer analysis, genetic research, or non-casework forensic applications. For additional rights, contact Promega Corp., Madison, WI, USA.

Specifications