Applied Biosystems™

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit

The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler Kit, eliminating자세히 알아보기

The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector and other non-FTA substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features of the AmpFLSTR Identifiler Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample type	Success Rate (50 rfu Threshold)	Success Rate (150 rfu Threshold)	Number of Samples Tested
Blood on FTA Card	99.4-100% Mean: 99.5%	95.7-98.8% Mean: 97.3%	414
Buccal on FTA Card	91.8-99.4% Mean: 97.1%	84.2-95.5% Mean: 90.9%	643
Buccal on Bode Buccal DNA Collector Card	97.5-98.8% Mean: 97.8%	88.1-98.8% Mean: 94.0%	582

Table 1: As part of its rigorous development, the Identifiler Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess “first pass” success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA Cards and Buccal DNA Collectors were amplified with the Identifiler Direct Kit, and the resulting data was analyzed using GeneMapper ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler Direct Kit is part of a new generation of AmpFLSTR Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR STR Kits, instrumentation, software, and consumables.

The Identifiler Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler Direct Kit should perform its own internal validation studies.

The Identifiler Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

사양

형식Kit

중합효소AmpliTaq Gold DNA Polymerase

기술STR (Short Tandem Repeat) Analysis

용도(장비)310 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, GeneAmp 9600, GeneAmp 9700, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer

라벨 또는 염료FAM, LIZ, NED, PET, VIC

매트릭스 및 크기 표준GeneScan 500 LIZ Size Standard, Matrix Standard Set DS-33

제품라인AmpFLSTR, Identifiler

제품 유형Direct PCR Amplification Kit

수량1000 Reactions

배송 조건Dry Ice

타겟 위치D21S11, CSF1PO, vWA, D8S1179, TH01, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D7S820, D13S317, D19S433

용도(애플리케이션)STR-based Human Identification

반응 형식SuperMix or Master Mix

샘플 종류Blood, Blood Samples, DNA (Genomic), Forensic Sample, Saliva, Whatman FTA Cards Samples

크기(최종 제품)<360 bp

부피25 μL⁄reaction

Unit SizeEach