Thermo Fisher Scientific SIRPA Monoclonal Antibody (OTI7B3), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on myeloid cells such as granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase-coupled signaling pathways.
Binding of SIRP alpha to CD47 triggers phosphorylation of ITIMs, recruiting tyrosine phosphatases SHP-1 and SHP-2.
This interaction regulates processes such as inhibition of macrophage phagocytosis and activation of T cells and dendritic cells.
SIRP alpha influences cellular responses to serum, growth factors, insulin, oncogenes, and adhesion, playing roles in physiological and pathological processes.
Cancer cells overexpress CD47, activating SIRP alpha to inhibit macrophage-mediated destruction of tumor cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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