Thermo Fisher Scientific MCM5 Polyclonal Antibody
MCM5 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 친화 크로마토그래피로 정제되었습니다. 핵 내 MCM5 단백질 검출에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–230 of human MCM5 (NP_006730.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914497 |
Product Specific Information
- Positive test controls: HeLa, Jurkat, SW620, Mouse spleen, Rat thymus
- Subcellular localization: Nucleus
Immunogen sequence:
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ
Target Information
The MCM5 protein is structurally similar to the CDC46 protein from S. cerevisiae, which is involved in the initiation of DNA replication. It belongs to the MCM family of chromatin-binding proteins and interacts with other MCM family members. The protein is upregulated during the G0 to G1/S phase transition and plays a role in cell cycle regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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