Thermo Fisher Scientific TK1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Thymidine Kinase 1 (TK1). Validated for WB, IHC, ICC/IF, and ELISA. High purity via affinity chromatography, supplied in PBS with glycerol. Suitable for detecting TK1 in cytoplasmic localization across multipl...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 15–194 of human Thymidine Kinase 1 (NP_003249.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.69 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914500 |
Product Specific Information
Positive test controls include HeLa, 293T, Jurkat, Mouse liver, Mouse spleen, Mouse stomach, Rat liver, Rat spleen, and Rat stomach.
The target is typically localized in the cytoplasm.
Immunogen sequence:
SKTRGQIQVI LGPMFSGKST ELMRRVRRFQ IAQYKCLVIK YAKDTRYSSS FCTHDRNTME ALPACLLRDV AQEALGVAVI GIDEGQFFPD IVEFCEAMAN AGKTVIVAAL DGTFQRKPFG AILNLVPLAE SVVKLTAVCM ECFREAAYTK RLGTEKEVEV IGGADKYHSV CRLCYFKKAS
Target Information
Thymidine Kinase 1 (TK1) is a highly conserved phosphotransferase present in most living cells. It catalyzes the phosphorylation of deoxythymidine with ATP to form deoxythymidine 5'-phosphate and ADP, playing a key role in DNA synthesis. TK1 is essential for the action of antiviral drugs such as azidothymidine (AZT) and is used in hybridoma cell line selection during monoclonal antibody production. Clinically, TK1 serves as a marker for cell division activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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