Thermo Fisher Scientific SPARC/Osteonectin Monoclonal Antibody (OSTN/3758)
인간 SPARC/Osteonectin 단백질을 인식하는 마우스 단클론 항체입니다. Western blot, IHC(P) 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태로 제공됩니다. 4°C에서 안정하며, 24개월간 품질 유지가 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OSTN/3758 |
| Immunogen | Recombinant fragment (around aa1–200) of human SPARC protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is stable for 24 months.
- Positive Control: Human placenta, lymph node or spleen; JEG3 or A549 cell lysates.
- Cellular Localization: Secreted; Basement membrane.
Specificity Comments:
SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin, is a phosphorylated, acidic, glycine-rich glycoprotein secreted by endothelial cells. It is abundant in the parietal endoderm of mouse embryos and human placenta. SPARC plays a key role in bone calcification and is highly conserved across species. It can be selectively expressed by endothelial cells in response to injury, influencing endothelial barrier function through F-actin–dependent morphological changes that facilitate macromolecule extravasation.
Target Information
The SPARC gene encodes a cysteine-rich, acidic, matrix-associated protein essential for collagen calcification in bone. It also contributes to extracellular matrix synthesis and modulation of cell shape. SPARC has been linked to both tumor suppression and metastasis due to its effects on cell morphology. Three transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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