Thermo Fisher Scientific 6-His Tag Polyclonal Antibody
6x His 태그 단백질 검출용 항체로 Western blot, ICC, ELISA, IP에 적합. Rabbit polyclonal IgG 형식으로 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태이며, 4°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
This antibody does not detect all N-terminal 6-His tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly fused to recombinant proteins at either the N- or C-terminus to facilitate purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins lacking specific antibodies.
His-tagged proteins can be purified using Ni²⁺-NTA resin due to the strong and selective binding of histidine residues to nickel ions.
His tag-specific antibodies are widely used for detection, purification, and co-immunoprecipitation of tagged proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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