Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, HRP
6x His 태그 단백질 검출용 HRP 결합 토끼 폴리클로날 항체. Western blot, ICC/IF, ELISA에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용 전용 제품으로 4°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:200 – 1:500 |
| ELISA | 1:10,000 – 1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.05% Pro-Clean 400 |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Note: This antibody does not detect all N-terminal 6-His tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins by fusing a known epitope sequence. His-tagged proteins are purified efficiently due to the strong and selective chelation between histidine clusters and Ni²⁺ ions on Ni²⁺-NTA matrices.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of His-tagged proteins in various assays.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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