Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, FITC
FITC로 표지된 6x His 태그 폴리클로날 항체로, 면역세포 염색 및 단백질 검출에 적합합니다. 토끼 유래 IgG로 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 498/517 nm 형광 특성을 가지며, 4°C 암소에서 보관합니다.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100 – 1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH.
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
This antibody does not detect all N-terminal 6-His tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tags are commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable purification using immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins even when specific antibodies are unavailable.
His-tagged proteins, due to histidine’s metal-binding properties, can be purified efficiently using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are useful for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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