Thermo Fisher Scientific Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor 488, eBioscience
마우스 Nanog 단백질을 특이적으로 인식하는 Alexa Fluor 488 결합 단일클론 항체입니다. 배아줄기세포의 미분화 상태 확인에 유용하며, Flow cytometry 및 ICC/IF에 적합합니다. 488 nm 여기, 519 nm 방출 특성을 가지며 4°C 암소 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 7 |
| Immunohistochemistry (IHC) | - | 8 |
| Immunohistochemistry (PFA fixed) | - | 1 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 31 |
| Flow Cytometry (Flow) | 0.06 µg/test | 8 |
| ChIP assay (ChIP) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMLC-51 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499/520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763611 |
Additional Formats
- Unconjugated
- eFluor 660
- Request custom conjugation
Product Specific Information
The eBioMLC-51 monoclonal antibody recognizes mouse Nanog, a homeobox transcription factor crucial for maintaining pluripotency in embryonic stem cells, independent of LIF/Stat3. Nanog is expressed in early embryos, ICM of blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Its expression decreases upon differentiation, making it a key marker for stem cell pluripotency.
Nanog functions as a transcriptional activator with activation domains CD2 and WR in the C-terminus and one in the N-terminus. The CD2 domain is unique to Nanog, while the NK2 DNA-binding domain is conserved. Immunoblotting with eBioMLC-51 identifies a ~45 kDa band in F9 lysate, but not in NIH3T3 or mouse spleen lysates.
For intracellular staining of mouse Nanog, use the eBioscience Foxp3 Staining Buffer Set (Product #00-5523-00).
Applications reported include intracellular staining for flow cytometry and immunocytochemistry.
Tested Applications:
- Flow cytometry (≤0.06 µg/test)
- Immunocytochemistry (≤10 µg/mL on fixed, permeabilized F9 cells)
Recommended Excitation/Emission: 488 nm / 519 nm (Blue Laser)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NANOG (Nanog homeobox) is a homeodomain protein regulating pluripotency and differentiation in embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines and absent in differentiated cells. Human NANOG shares 52% amino acid identity with mouse NANOG (85% in homeodomain).
- Human NANOG: gene locus 12p13.31
- Mouse NANOG: gene locus 6 F2
NANOG expression is detected in the inner cell mass of blastocysts and is critical for ES cell self-renewal. Overexpression promotes S-phase entry and proliferation. It is also a key factor in generating induced pluripotent stem (iPS) cells alongside Oct4, Sox2, Klf4, Lin28, and c-Myc, offering potential applications in regenerative medicine.
For Research Use Only. Not for diagnostic use or resale without authorization.
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