Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 660, eBioscience
마우스 CD4 항원을 인식하는 GK1.5 단클론 항체로, eFluor 660 형광체로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.125 µg/test 농도로 사용 가능합니다. 633 nm 레이저로 여기되어 668 nm에서 방출됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 18 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.125 µg/test | 173 |
| Functional Assay | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596360 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 661, Brilliant UV 737, Brilliant Violet 480, Brilliant Violet 605, eFluor 450, eFluor 506, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436/600/645/702/780 등 다양한 형광체 버전 제공.
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays a crucial role in T cell development and function. In T cells, CD4 associates with the protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
- Fluorophore Information: eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited by the red laser (633 nm).
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD4 antigen is involved in MHC class II recognition and serves as a co-receptor for HIV. It is primarily expressed in T helper cells but also in monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. It plays a key role in T-cell activation through association with the T-cell receptor complex and kinase Lck, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known.
For Research Use Only. Not for use in diagnostic procedures.
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