Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 661, Brilliant UV 737, Brilliant Violet 480, Brilliant Violet 605, eFluor 450, eFluor 506, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436/600/645/702/780 등 다양한 형광체 버전 제공.

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays a crucial role in T cell development and function. In T cells, CD4 associates with the protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Fluorophore Information: eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited by the red laser (633 nm).
• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD4 antigen is involved in MHC class II recognition and serves as a co-receptor for HIV. It is primarily expressed in T helper cells but also in monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. It plays a key role in T-cell activation through association with the T-cell receptor complex and kinase Lck, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known.

For Research Use Only. Not for use in diagnostic procedures.

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