Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 800
GFP 단백질을 인식하는 DyLight 800 결합 염소 폴리클로날 항체로, Western blot, ICC/IF, ELISA에 적합합니다. 고감도 형광 검출이 가능하며, 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:20,000–1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein (full length, 246aa) derived from Aequorea victoria |
| Conjugate | DyLight™ 800 |
| Excitation/Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only before immediate use.
Assay results:
Immunoelectrophoresis showed a single precipitin arc against anti-Goat Serum and purified GFP (Aequorea victoria).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria emits green light (508 nm) upon excitation with blue light (395 nm). GFP is widely used as a reporter for gene expression and as a fusion tag for protein localization and dynamics studies in both eukaryotic and prokaryotic systems. It forms a stable, autocatalytic fluorescent pigment without requiring cofactors or substrates. GFP fluorescence is species-independent and suitable for diverse applications, including FRET and yeast two-hybrid assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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