Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 405
DyLight 405 형광 표지를 가진 Thermo Fisher Scientific의 GFP 폴리클로날 항체로, Western blot, ICC/IF, ELISA 등 다양한 응용에 적합합니다. 염소 유래 IgG 항체이며, GFP 태그 단백질 검출에 특화되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:20,000–1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full-length amino acid sequence (246aa) derived from Aequorea victoria |
| Conjugate | DyLight™ 405 |
| Excitation / Emission Max | 400 / 423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if solution is not clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified GFP (Aequorea victoria). No reaction was observed against Human, Mouse, or Rat serum proteins.
Target Information
The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light during bioluminescence. GFP is widely used as a reporter protein for gene expression and as a protein tag in both eukaryotic and prokaryotic systems. It enables localization studies, protein movement tracking, and subcellular compartment analysis.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm). GFP fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid system), and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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