Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 436, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 436, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 2 publications

Product Specifications

Additional Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Recommended usage ≤ 0.5 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. A 450/50 bandpass filter or equivalent is recommended. Verify instrument compatibility before use.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

This protein is an MHC class I alloantigen, involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are key tools in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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