Thermo Fisher Scientific IgD Monoclonal Antibody (11-26c (11-26)), Super Bright 436, eBioscience
마우스 IgD 델타 중쇄를 인식하는 단클론 항체로, Super Bright 436 형광체 결합. Flow Cytometry에 최적화되어 있으며, 비특이적 반응 최소화를 위해 Super Bright Staining Buffer 사용 권장. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 1 publication |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11-26c (11-26) |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413/431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762758 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin, including IgA, IgG, or IgM. IgD is expressed by peripheral mature B cells, and 11-26c does not activate B cells.
Applications Reported:
This antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.5 µg per test (100 µL final volume). Optimal concentration should be titrated depending on cell number (10⁵–10⁸ cells/test).
Fluorochrome Information:
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent. Ensure the instrument supports this fluorochrome.
Staining Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fluorescence Parameters:
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
IgD is a type I transmembrane protein co-expressed with membrane IgM on mature naive B cells and also exists in soluble form in serum. It associates with the B cell receptor and participates in signal transduction upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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