Thermo Fisher Scientific IgE Monoclonal Antibody (Ige21), Super Bright 436, eBioscience
인간 IgE를 특이적으로 인식하는 마우스 단클론 항체로, Super Bright 436 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 비특이적 결합을 최소화하는 Super Bright Staining Buffer와 함께 사용 권장. 405 nm 레이저로 여기되어 436 nm에서 방출.
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Thermo Fisher Scientific IgE Monoclonal Antibody (Ige21), Super Bright 436, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Ige21 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413/431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744814 |
Additional Formats
- APC
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- Super Bright 600
- Super Bright 780
- Request custom conjugation available
Product Specific Information
The monoclonal antibody IgE21 recognizes the immunoglobulin E (IgE) class of antibodies. IgE levels in serum are low and play a key role in allergic responses by binding to mast cells and basophils via the Fc epsilon receptor I, leading to histamine release. IgE is produced by plasma B cells and basophils and exists in both soluble and membrane-bound forms, both recognized by IgE21.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume). Cell counts can range from 10⁵–10⁸ cells/test.
Super Bright 436 is excited at 405 nm and emits at 436 nm. Recommended detection filter: 450/50 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and compensation.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
IgE is one of five immunoglobulin classes in mammals, consisting of two heavy epsilon chains and two light chains. It plays a central role in type I hypersensitivity reactions (e.g., hay fever, asthma, hives, anaphylaxis) by binding to Fc receptors on mast cells and basophils. Crosslinking of IgE induces degranulation, promoting allergic responses. IgE also contributes to parasite immunity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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