Thermo Fisher Scientific ULBP1 Polyclonal Antibody
Human ULBP1 단백질을 인식하는 Goat Polyclonal Antibody로 Western blot 및 Neutralization assay에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS와 5% trehalose buffer에 동결건조 상태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Neutralization (Neu) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sf21-derived recombinant human ULBP-1 (Gly26–Pro215) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605619 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human ULBP-2 and ULBP-3 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU/µg of antibody (LAL method).
Target Information
ULBP1 is a ligand for the NKG2D receptor, along with ULBP2 and ULBP3. These ligands activate multiple signaling pathways in primary NK cells, leading to cytokine and chemokine production.
Binding of ULBP ligands to NKG2D induces calcium mobilization and activation of JAK2, STAT5, ERK, and PI3K/Akt pathways.
In CMV-infected cells, ULBP1 interacts with soluble CMV glycoprotein UL16, which blocks interaction with NKG2D, allowing viral immune evasion. UL16 also retains ULBP1 in the ER and cis-Golgi, preventing its expression on the cell surface.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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