Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor 488, eBioscience
Alexa Fluor 488으로 표지된 SSEA-1 단클론 항체로, 마우스 및 인간 세포에서 발현되는 CD15 항원을 검출. Flow cytometry에 최적화되어 있으며, 세포 분화 및 접착 연구에 활용 가능. 4°C에서 암소 보관, 동결 금지.
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Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 12 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Human |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-480 (MC-480) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596519 |
Available Formats
- Unconjugated
- Biotin
- eFluor 660
- PE
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), embryonic stem cells (ES), and murine/human germ cells (EG).
No immunoreactivity is observed with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse it decreases.
SSEA-1 is also found on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of F9 cells at 5 µL (0.125 µg) per test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD15 (Lewis X, Le(x); SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types, including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. High surface expression promotes calcium-dependent Le(x)-Le(x) interactions involved in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 is widely used in the diagnosis of Hodgkin’s disease.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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