Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor 488, eBioscience
SSEA-3 항원을 인식하는 Rat IgM 형 단일클론 항체로, Alexa Fluor 488 형광 표지. 인간 및 마우스 세포에 반응하며, Flow Cytometry용으로 최적화됨. 인간 ES, EG, EC 세포에서 발현되는 표면 마커 분석에 적합.
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Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor 488, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (1 µg)/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rat / IgM |
| Recommended Isotype Control | Rat IgM Isotype Control (eBRM), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-631 (MC-631) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10732357 |
Additional Formats
Product Specific Information
The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES), and is downregulated following differentiation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated on the 2102Ep cell line. Use at 5 µL (1 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SSEA-3 (Stage-specific embryonic antigen 3) is a cell surface antigen expressed along with SSEA-4, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-induced differentiation.
Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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