Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Alexa Fluor 488, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1 µg)/test
Publications: View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Product Specific Information

The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES), and is downregulated following differentiation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated on the 2102Ep cell line. Use at 5 µL (1 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

SSEA-3 (Stage-specific embryonic antigen 3) is a cell surface antigen expressed along with SSEA-4, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-induced differentiation.
Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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