Thermo Fisher Scientific GFP Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: Recombinant or native GFP

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.
GFP has been widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or investigate the dynamics of subcellular compartments where these proteins are targeted.

GFP technology has provided new insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment.
The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without the need for additional proteins, substrates, or cofactors.

GFP fluorescence is stable, species-independent, and suitable for various applications.
It is extensively used as a fluorescent tag to monitor gene expression and protein localization.
Other applications include assessing protein–protein interactions in yeast two-hybrid systems and measuring distances between proteins in fluorescence resonance energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.