Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody
DYKDDDDK(FLAG) 태그 단백질 검출용 Rabbit Polyclonal 항체. Western blot에 최적화된 고감도 항체로, 단백질 정제 및 발현 분석에 활용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide of DYKDDDDK conjugated to a carrier protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: FLAG tagged fused proteins
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
It is typically fused to the N- or C-terminus of a protein of interest, enabling easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including protein-protein interaction studies, protein expression analysis, and localization studies.
Its hydrophilic nature minimizes interference with protein folding or activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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