Thermo Fisher Scientific Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), PE, eBioscience
Myeloperoxidase(MPO)를 인식하는 단클론 항체로, 인간 세포 내 염색 및 유세포분석에 최적화됨. PE 형광 표지로 565/576 nm에서 검출 가능. 친화 크로마토그래피로 정제되었으며, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 4 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Bovine, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MPO455-8E6 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10733265 |
Product Specific Information
The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO consists of two 55 kDa and two 15 kDa subunits; the larger subunits contain a heme group giving a greenish color. As an enzyme, MPO decomposes hydrogen peroxide and oxidizes tyrosine, producing hypochlorous acid and tyrosyl radicals responsible for neutrophil cytotoxic activity. MPO is clinically relevant for cancer diagnosis and correlates with cardiac events.
Applications Reported:
This antibody is used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for intracellular staining of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
For optimal results:
- Stain whole blood with surface antigen antibodies.
- Treat with 1X RBC lysis buffer (Product # 00-4333-57).
- Fix cells with IC Fixation Buffer (Product # 00-8222-49).
- Wash twice with Permeabilization Buffer (Product # 00-8333-56).
- Incubate with MPO455-8E6 for 30–60 minutes.
After washing, analyze on a flow cytometer. Use 5 µL (0.5 µg) per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test.
Optical Properties:
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It forms a tetramer of two light and two heavy chains. MPO generates hypohalous acids essential for neutrophil microbicidal activity. Associated diseases include Myeloperoxidase Deficiency and Familial Alzheimer’s Disease type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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