Thermo Fisher Scientific Cathepsin F Polyclonal Antibody
Thermo Fisher Scientific의 Cathepsin F Polyclonal Antibody는 마우스 Cathepsin F 단백질을 인식하는 염소 유래 IgG 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 높은 특이성과 낮은 교차 반응성을 제공합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin F (Ser20–Asn462) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576799 |
Product Specific Information
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Cathepsin C, Cathepsin H, and Cathepsin L is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. They generally contain a signal sequence, followed by a propeptide and a catalytically active mature region.
Cathepsin F precursor has a long proregion containing a cystatin-like domain, unique within the papain family. This domain shares structural similarities with cysteine protease inhibitors of the cystatin superfamily.
CTSF encodes a 484 amino acid protein with a 19-residue signal peptide. Cathepsin F contains five potential N-glycosylation sites and may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The gene is ubiquitously expressed and maps to chromosome 11q13, near the cathepsin W gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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