Thermo Fisher Scientific alpha-Fetoprotein Monoclonal Antibody (1E8), eBioscience
인간 알파태아단백질(AFP)을 인식하는 단클론 항체로, Western blot, IHC, ICC에 적합합니다. 간세포암 및 생식세포종양 등의 연구용 마커로 활용됩니다. 고순도(>90%) 항체로, 0.5 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865385 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP), a 70-kDa secretory protein in the albumin gene family. AFP is synthesized by the yolk sac and fetal liver during embryogenesis, with the highest levels found in fetal serum. After birth, AFP levels decrease dramatically and are nearly undetectable in normal adult serum.
Elevated AFP levels in adults can indicate hepatocellular carcinoma, germ cell teratoblastoma, liver regeneration, viral hepatitis, or cirrhosis. Therefore, AFP detection is widely used as a diagnostic marker for these conditions.
Applications Reported:
This 1E8 antibody has been reported for use in:
- Western blotting
- Immunohistochemistry (formalin-fixed paraffin-embedded tissue sections)
- Immunocytochemistry
Applications Tested:
Tested by Western blot on reduced HepG2 cell lysate and by IHC on FFPE human liver section using high pH antigen retrieval. Recommended use ≤5 µg/mL, with titration advised for optimal performance.
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma, though hereditary persistence can occur without pathology. AFP is considered the fetal counterpart of serum albumin, with both genes located on chromosome 4.
AFP exists in monomeric, dimeric, and trimeric forms and can bind copper ions, nickel ions, fatty acids, and bilirubin. AFP levels in amniotic fluid are used to screen for neural tube defects such as spina bifida and anencephaly. Elevated serum AFP levels have been observed in hepatocellular carcinoma, teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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