Thermo Fisher Scientific CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience
CD271 (NGF Receptor) 단일클론 항체 ME20.4는 인간 및 다양한 종의 CD271을 인식하며, 신경세포 및 줄기세포 연구에 적합합니다. Flow cytometry, IHC, WB 등 다양한 응용 가능. 고순도(>90%) 및 안정적 액상 형태로 제공.
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Thermo Fisher Scientific CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.25 µg/test | View 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat |
| Published Species | Dog, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ME20.4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865377 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
- Alexa Fluor 488
- APC
- PE
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein belonging to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and certain lymphoid cells including follicular dendritic cells.
CD271 binds to NGF, BDNF, NTF3, and NTF4, and contains a death domain that induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.
The ME20.4 antibody recognizes CD271 from human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig.
Applications Reported: Flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested:
- Flow cytometric analysis of SK-N-SH cells (≤0.25 µg/test; 100 µL final volume; 10^5–10^8 cells/test)
- Immunohistochemistry of formalin-fixed paraffin-embedded human tissue using high pH antigen retrieval (≤5 µg/mL)
- Antibody titration recommended for optimal assay performance.
Target Information
Neurotrophic factors regulate the survival, differentiation, and maintenance of neurons and neural crest-derived cells. NGF is essential for developing sympathetic neurons during target competition in vivo. Antibodies specific to NGFR p75 are valuable tools for detecting and characterizing NGFR p75, aiding research on neural development, plasticity, and repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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