Thermo Fisher Scientific GAPDH Monoclonal Antibody (FF26A), eBioscience
GAPDH 단백질을 검출하기 위한 FF26A 단일클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot 및 면역침강(IP)에 사용 가능하며, 단백질 로딩 컨트롤로 활용됩니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 9 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FF26A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151704 |
Product Specific Information
This FF26A monoclonal antibody reacts with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme in glycolysis. GAPDH (36 kDa) is constitutively expressed in all tissues at varying levels and is commonly used as a control for protein loading in differential expression experiments.
The FF26A antibody is cross-reactive to mouse GAPDH.
- Applications Reported: Western blotting
- Applications Tested: Immunoblotting of cell lysates (≤1 µg/mL). Antibody titration recommended for optimal performance.
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, forming a tetramer of identical 37 kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It plays essential roles in apoptosis, neurodegenerative diseases (e.g., Alzheimer’s, Huntington’s), and cancer-related cellular processes.
Due to its high expression across tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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