Thermo Fisher Scientific IDO1 Monoclonal Antibody (OTI2A10), TrueMAB
IDO1 단백질을 인식하는 Mouse Monoclonal Antibody (Clone OTI2A10)로, Western blot 및 Immunocytochemistry에 적합합니다. HEK293T 세포에서 발현된 full-length human IDO1을 면역원으로 사용하였으며, carrier-free 형태로 공급됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A10 |
| Immunogen | Full length human recombinant protein of human IDO1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) prior to use.
Target Information
Indoleamine 2,3-dioxygenase 1 (IDO1) is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of tryptophan, serotonin, and melatonin, leading to the production of kynurenines.
IDO1 is expressed in various human tissues, macrophages, and dendritic cells, and is induced by interferons (IFNs).
It plays a key role in antimicrobial defense by depleting tryptophan, thereby inhibiting the growth of viruses, bacteria, and parasites.
Additionally, IDO1 is involved in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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