Thermo Fisher Scientific TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), FITC, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.125 µg/test
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Product Specifications

Product Specific Information

The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on other T cell hybridomas tested. The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d^ major histocompatibility molecules. The use of DO11.10 and KJ1-26 has been instrumental in describing many details of T cell immunity.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of the DO11.10 T cell hybridoma. Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Fluorochrome Information:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas. The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323–339) in the context of I-A^d^ major histocompatibility molecules. Antibodies against DO11.10 have been key in elucidating mechanisms of T cell activation, development, and function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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