Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (OTI5D11), TrueMAB
EIF4E2 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, Western blot 및 Immunocytochemistry에 적합. HEK293T 세포에서 생산된 full-length 인간 EIF4E2 단백질을 면역원으로 사용. 동결건조 형태이며, PBS buffer(8% trehalose)로 보존. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D11 |
| Immunogen | Full length human recombinant protein of human EIF4E2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349).
Acts as a repressor of translation initiation (PubMed:22751931).
Unlike EIF4E, it cannot bind eIF4G (EIF4G1, EIF4G2, or EIF4G3), suggesting it competes with EIF4E and blocks assembly of eIF4F at the cap. [UniProt]
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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