Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (OTI1C1), TrueMAB
EIF4E2 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(P) 검증됨. Lyophilized 형태이며 PBS buffer(pH 7.3, 8% trehalose)로 구성. 연구용으로 translation initiation 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C1 |
| Immunogen | Full length human recombinant protein of human EIF4E2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform another round of desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in translation initiation.
Acts as a repressor of translation initiation and is unable to bind eIF4G (EIF4G1, EIF4G2, or EIF4G3), suggesting competition with EIF4E to block assembly of eIF4F at the cap.
[UniProt references: PubMed 9582349, 17368478, 25624349, 22751931]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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