Thermo Fisher Scientific Phospho-Tau (Ser199, Ser202) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide phosphorylated at serines 199 and 202.

The positive control used in western blotting was recombinant human Tau or African green monkey kidney (CV-1) cells stably expressing human four-repeat tau and SV40 small T antigen to induce specific inhibition of PP2A.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker protein. Axonal polarity is predetermined by TAU/MAPT localization.

Hyper-phosphorylated Tau forms paired helical filaments (PHF), the major component of neurofibrillary lesions in Alzheimer’s disease brain. This modification impairs Tau’s microtubule binding, leading to neuronal degeneration. Tau is phosphorylated by several kinases, including GSK-3β, PKA, CDK5, and casein kinase II. Hyper-phosphorylated Tau is also found in Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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