Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test
  View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is upregulated by the presence of IgE. It forms a tetrameric complex with one β and two γ subunits, which possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.25 µg) per test in a final volume of 100 µL.
Cell number can range from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Ensure instrument compatibility.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.

Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

FceR1 (Fc epsilonR1 alpha):
A subunit of the high-affinity receptor of IgE, forming a tetrameric complex with one α, one β, and two γ subunits. The β and γ subunits mediate signal transduction. This receptor is key in triggering allergic responses such as hay fever and asthma. Crosslinking of IgE-bound FcεRI receptors by allergens leads to release of histamine and other mediators, initiating inflammatory responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지