Thermo Fisher Scientific TACC3 Monoclonal Antibody (OTI3B1), TrueMAB
TACC3 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, PBS 버퍼에 trehalose 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific TACC3 Monoclonal Antibody (OTI3B1), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B1 |
| Immunogen | Full-length human recombinant TACC3 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TACC1 is located on the 8p11 chromosomal region, amplified in about 15% of breast tumor samples. This region also contains FGFR1, which is overexpressed in most breast cancer tumors.
TACC family members (TACC1, TACC2, TACC3) are closely linked to FGFR genes (FGFR1, FGFR2, FGFR3) on chromosomes 4, 8, and 10, respectively. Due to their phylogenetic relationship, TACC and FGFR are proposed to have similar roles in cell growth and differentiation.
TACC1 contains a conserved C-terminal region similar to the Drosophila homolog D-TACC, which is essential for normal spindle function. Mammalian TACC proteins interact with centrosomes and microtubules in a similar manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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