
Thermo Fisher Scientific Ub-K63 Recombinant Rabbit Monoclonal Antibody (JM09-67)
Ub-K63 특이적 결합을 가지는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF에 적합. 높은 특이성과 재현성, 로트 간 일관성 제공. 단백질 분해 및 유비퀴틴 경로 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM09-67 |
| Immunogen | Synthetic peptide within Human K63-linkage Specific Ubiquitin (aa 36–85) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809850 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Ubiquitin is a conserved 76 amino acid polypeptide involved in proteasomal degradation and post-translational modification processes.
The ubiquitin pathway regulates cellular growth and proliferation through covalent attachment of ubiquitin to lysine residues on target proteins. This process involves three enzymatic steps mediated by E1 (activating enzyme), E2 (conjugating enzyme), and E3 (ligase), which transfer ubiquitin to substrate proteins for degradation or signaling regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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