Thermo Fisher Scientific NUMB Recombinant Rabbit Monoclonal Antibody (JM10-023)
NUMB 단백질 검출용 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC에 사용 가능. 높은 특이성과 민감도, 로트 간 일관성 제공. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-023 |
| Immunogen | Synthetic peptide within Human NUMB aa 4–47 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809845 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages:
- Excellent specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Protein NUMB homolog is a conserved protein that regulates differentiation of neuronal progenitor cells. It functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is expressed in postmitotic neurons and contributes to axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain show extensive neurite outgrowth, which can be inhibited by voltage-gated Ca²⁺ channel inhibitors or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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