Thermo Fisher Scientific GNAI3 Polyclonal Antibody
Thermo Fisher Scientific의 GNAI3 Polyclonal Antibody는 인간 및 마우스 시료에 반응하며, Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 고순도 친화 크로마토그래피 정제 항체로, 세포막 및 세포질 내 GNAI3 단백질 검출에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–354 of human GNAI3 (NP_006487.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.41 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805389 |
Product Specific Information
Immunogen sequence:
MGCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV KQMKIIHEDG YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR ADDARQLFVL AGSAEEGVMT PELAGVIKRL WRDGGVQACF SRSREYQLND SASYYLNDLD RISQSNYIPT QQDVLRTRVK TTGIVETHFT FKDLYFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF DSICNNKWFT ETSIILFLNK KDLFEEKIKR SPLTICYPEY TGSNTYEEAA AYIQCQFEDL NRRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKE CGLY
Positive Samples: LO2, MCF-7, HL-60, HT-1080
Cellular Location: Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Centrosome, Cytoskeleton, Microtubule organizing center
Target Information
Heterotrimeric G proteins relay signals from cell surface receptors to intracellular effectors. Each receptor detects specific extracellular stimuli such as photons, hormones, or neurotransmitters. Effectors like adenyl cyclase generate intracellular messengers. Mammalian G proteins consist of alpha, beta, and gamma subunits encoded by at least 16, 4, and 7 genes, respectively. The alpha subunits bind and hydrolyze GTP, regulating effector activity. Four main classes of Galpha subunits exist: Gs, Gi, Gq, and Galpha 12/13. The Gi class includes Galpha i-1, i-2, i-3, o, t1, t2, z, and gust, which are pertussis toxin-sensitive and function to open atrial potassium channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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