Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), Functional Grade, eBioscience

Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• Custom conjugation available upon request

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests its role in inflammatory responses.

Applications Reported:

• Flow cytometric analysis
• Blocking of CD106 in functional studies

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended use ≤0.25 µg per test (100 µL final volume). Optimal antibody titration is advised for best assay performance.

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtered

Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration and adhesion, and may play a role in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.